I am planning to use CRISPR knock-in to add GFP to the C-terminus of the SPDEF gene in the T-47D cell line. Before proceeding, I want to check the gene copy number of SPDEF to assess the likelihood of obtaining homozygous clones.
In the DepMap database, I found two different results: the Copy Number (Absolute) database reports 3 copies, while the Copy Number Public 24Q2 (Log2 transformed) indicates 2 copies. I’m confused about which one accurately reflects the gene copy number.
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